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From DNA-sampling robots and invasive species maps to genetic libraries and mosquito birth control, USGS scientists are hard at work building up ecological infrastructure for the nation.

The Aquatic eDNAtlas Project supports effective conservation and management of freshwater biota. Rocky Mountain Research Station scientists have been at the forefront of investigating and applying eDNA sampling for aquatic species.

The eDNAtlas allows land managers, scientists, and the public to access results from samples collected from aquatic systems throughout the U.S. The eDNAtlas database contains results from thousands of sites.

Environmental DNA (eDNA) has gone from new science on the block to a star of the show. With just a water, soil, or snow sample, ecologist Tommy Franklin and fellow scientists at the Rocky Mountain Research Station are detecting rare aquatic and terrestrial species by identifying the genetic material they leave behind.

Scientists at the Rocky Mountain Research station recently used eDNA to preserve headwater bull trout populations in a warming climate, track white nose syndrome — a killer bat fungus, and reveal the role of human transport in the spread of invasive northern pike in the Columbia River basin. A recent article highlights eDNA research at the station and features National Genomics Center Director Mike Schwartz.

See also: Quagga and Zebra Mussels for more information

Assays of environmental DNA — traces of genetic material found in air, soil, and water — may improve scientists’ ability to detect bat roosts and track the spread of white-nose syndrome, a disease caused by a killer fungus spread easily in the close quarters of hibernacula. In this research, scientists experienced success in detecting bats in field collected samples of environmental DNA, though the technology is still in the experimental stage.

United States Geological Survey Science is Refining eDNA Techniques and Developing New Applications. Research by the U.S. Geological Survey is leading to new techniques in the use of environmental DNA, or eDNA, to detect invasive species and their spread.

Invasive zebra and quagga mussels are an immediate threat to Western states. With no controls, they spread rapidly, foul boats and equipment, clog water intake, and increase costs to hydropower operations and municipal water utilities. Even dead mussels can be a nuisance, littering beaches with shells. Management of these invaders is expensive; in the Great Lakes, managing mussels costs about $500 million annually. Until 2007, the mussels were limited to waterways and lakes east of the Mississippi, but now they have spread westward. In 2016, quagga mussels were found in Lake Powell.

Unfortunately, there are no foolproof existing technologies or treatments to eradicate established mussel populations in large, open water systems in an environmentally sound manner. Early warning, however, helps us prepare before the mussels or other invasive species arrive.

Tracking the establishment and spread of existing and new invasive species is critical to effectively manage invasive species. In addition to standard means of monitoring, the USGS is developing new tools, particularly molecular techniques, to assist in the early detection of invasive species. Provides research information about eDNA and Genetic Tools for Zebra and Quagga Mussels.

Environmental DNA methods are highly sensitive and accurate, making them ideal for detecting animals at low densities. However, this tool also comes with its own unique set of challenges when applied to efforts to eradicate invasive species. This research explores the use of eDNA for evaluating invasive species eradication efforts in streams and offers best practices for incorporating eDNA methods into invasive species removal projects.
Related Publication:
K. J. Carim, N. J. Bean, J. M. Connor, W. P. Baker, M. Jaeger, M. P. Ruggles, K. S. McKelvey, T. W. Franklin, M. K. Young, M. K. Schwartz. 2020. Environmental DNA sampling informs fish eradication efforts: Case studies and lessons learned

READI-Net is a network of robotic environmental DNA samplers to enhance the early detection of aquatic biological threats.

A framework from Rocky Mountain Research Station scientists can help managers decide if, when, and how to use eDNA sampling technology for invasive species monitoring or surveillance.

Citation: Morisette, Jeffrey; Burgiel, Stas; Franklin, Thomas; Wilcox, Taylor. 2022. Shedding light on shedded cells: Using eDNA sampling for surveillance of invasive species. Connected Science. Fort Collins, CO: U.S. Department of Agriculture, Rocky Mountain Research Station. 2 p.

Invasive species surveillance programs can utilize environmental DNA sampling and analysis to provide information on the presence of invasive species. Wider utilization of eDNA techniques for invasive species surveillance may be warranted. This paper covers topics directed towards invasive species managers and eDNA practitioners working at the intersection of eDNA techniques and invasive species surveillance. It provides background information on the utility of eDNA for invasive species management and points to various examples of its use across federal and international programs.

Citation: Morisette, J., S. Burgiel, K. Brantley, et al. 2021. Strategic considerations for invasive species managers in the utilization of environmental DNA (eDNA): Steps for incorporating this powerful surveillance tool. Management of Biological Invasions. 12(3): 747-775.

Northern pike are native to Alaska, Canada, and much of the central and eastern continental United States. Genetic evidence reveals the role of human transport in the spread of invasive northern pike in the Columbia River basin.

Natural resource managers in British Columbia discovered several adult male and female European green crabs on Haida Gwaii this past July. Alarm bells immediately went off for biologists in Alaska. The archipelago of Haida Gwaii, off the coast of Prince Rupert in British Columbia, is very close to Alaska. The July discovery is the closest confirmed finding of the invasive crustacean since it was first detected in the San Francisco Bay area in 1989.